'ESI-MASS'에 해당되는 글 1건
2007. 3. 24. 05:09
[카테고리 없음]
When people purify their proteins, they measure the molecular weight using MASS Spec. ESI-MASS is very nice to measure the molecular weight of proteins, and sometimes you find out your protein's MASS result is different from the theoretical molecular weight.
In most cases, unless the gene is correct, your proteins might be modified after translation. Post-translational modification is pretty common in any organisms, especially in eukayotes. Even if you express your protein in E. coli, you may get somewhat different MASS results.
The web site below is very useful to identify what kind of modification was occurred in your protein from molecular weight difference.
http://www.abrf.org/index.cfm/dm.home
If you want to see all kinds of modifications,
In most cases, unless the gene is correct, your proteins might be modified after translation. Post-translational modification is pretty common in any organisms, especially in eukayotes. Even if you express your protein in E. coli, you may get somewhat different MASS results.
The web site below is very useful to identify what kind of modification was occurred in your protein from molecular weight difference.
http://www.abrf.org/index.cfm/dm.home
If you want to see all kinds of modifications,
http://www.abrf.org/index.cfm/dm.home?AvgMass=all
| Avg.Mass Change |
Modification |
 | |
| -79 | 5' dephospho |
| -58 | Desmosine (from Lysine) |
| -48 | decomposed carboxymethylated Methionine |
| -44 | decarboxylation of gamma carboxy Glutamate |
| -43 | gamma-glutamyl semialdehyde (from arginine) |
| -42 | Ornithine (from Arginine) |
| -34 | Lysinoalanine (from Cysteine) |
| -34 | Lanthionine (from Cysteine) |
| -34 | Dehydroalanine (from Cysteine) |
| -30 | Homoserine formed from Met by CNBr treatment |
| -27 | Oxidation of arginine (to glutamic acid) |
| -18 | Formylglycine (from cysteine) |
| -18 | Pyroglutamic Acid formed from Glutamic Acid |
| -18 | Dehydration (-H2O) |
| -18 | S-gamma-Glutamyl (crosslinked to Cysteine) |
| -18 | O-gamma-Glutamyl- (Crosslink to Serine) |
| -18 | Serine to Dehydroalanine |
| -18 | Alaninohistidine (Serine crosslinked to theta or pi carbon of Histidine) |
| -18 | Misincorporation of Norleucine for Methionine |
| -18 | Succinimide formation from aspartic acid |
| -17 | Pyroglutamic Acid formed from Gln |
| -17 | N-pyrrolidone carboxyl (N terminus) |
| -17 | N alpha -(gamma-Glutamyl)-lysine |
| -17 | N-(beta-Aspartyl)-Lysine (Crosslink) |
| -17 | Succinimide formation from asparagine |
| -17 | S-carbamoylmethylcysteine cyclization (N-terminus) |
| -16 | Pyruvoyl- (Serine) |
| -5 | Crosslink between Arg and His sidechains |
| -4 | 3,3',5,5'-TerTyr (Crosslink) |
| -2 | Formylglycine (from serine) |
| -2 | Disulphide bond formation (Cystine) |
| -2 | S-(2-Histidyl)- (Crosslinked to Cysteine) |
| -2 | S-(3-Tyr) (Crosslinked to Cysteine) |
| -2 | 3,3'-BiTyr (Crosslink) |
| -2 | IsodiTyr (Crosslink) |
| -1 | Allysine (from Lysine) |
| -1 | Amide formation (C terminus) |
| -1 | Oxidation of lysine (to aminoadipic semialdehyde) |
| 1 | Deamidation of Asparagine and Glutamine to Aspartate and Glutamate |
| 1 | Citruline (from Arginine) |
| 2 | Cysteine x2, reduction of Cystine (Cys-Cys) |
| 2 | Reduction of indole double bond of Trp |
| 4 | Oxidation of Trp to kynurenine |
| 12 | Lysine epsilon amino to imine |
| 12 | Cysteine (N-term) formaldehyde adduct (Cys to Thioproline conversion) |
| 12 | Formaldehyde adduct of Trp |
| 13 | Syndesine (from Lysine) |
| 13 | CM-Cys vs PAM-Cys |
| 14 | Methylation (N terminus, N epsilon of Lysine, O of Serine, Threonine or C terminus, N of Asparagine) |
| 14 | CAM-Cys vs PAM-Cys |
| 15 | delta-Hydroxy-allysine (from Lysine) |
| 15 | Oxidation of lysine (to aminoadipic acid) |
| 16 | Hydroxylation (of delta C of Lysine, beta C of Tryptophan, C3 or C4 of Proline, beta C of Aspartate) |
| 16 | Oxidation of Methionine (to Sulphoxide) |
| 16 | 3,4-Dihydroxy-Phenylalanine (from Tyrosine) (DOPA) |
| 16 | Oxohistidine (from histidine) |
| 16 | Sulfenic Acid (from Cysteine) |
| 16 | Oxidation of proline (to gamma-glutamyl semialdehyde) |
| 22 | Sodium |
| 28 | Ethyl |
| 28 | N,N dimethylation (of Arginine or Lysine) |
| 28 | 2,4-BisTrp-6,7-dione (from Tryptophan) |
| 28 | Formylation (CHO) |
| 30 | 6,7 Dione (from Tryptophan) |
| 32 | 3,4,6-Trihydroxy-Phenylalanine (from Tyrosine) (TOPA) |
| 32 | 3,4-Dihydroxylation (of Proline) |
| 32 | Oxidation of Methionine (to Sulphone) |
| 32 | Oxidation of proline (to glutamic acid) |
| 32 | Double oxidation of Trp |
| 34 | 3-Chlorination (of Tyrosine with 35Cl) |
| 36 | 3-Chlorination (of Tyrosine with 37Cl) |
| 38 | Potassium |
| 42 | Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) |
| 42 | N-Trimethylation (of Lysine) |
| 43 | Carbamylation |
| 44 | disodium |
| 45 | Nitro (NO2) |
| 46 | beta-Methylthio-aspartic acid |
| 48 | Cysteic acid, oxidation of cysteine |
| 51 | Piperidine adduct to C-terminal Cys |
| 56 | t-butyl ester(OtBu) and t-butyl (tBu) |
| 57 | Glycyl (-G-,-Gly-) |
| 57 | Carboxamidomethyl (on Cysteine) |
| 58 | Carboxymethyl (on Cysteine) |
| 60 | sodium + potassium |
| 64 | Selenocysteine (from Serine) |
| 67 | Asp transamidation with piperidine |
| 68 | 3,5-Dichlorination (of Tyrosine with 35Cl) |
| 69 | Dehydroalanine (Dha) |
| 70 | 3,5-Dichlorination (of Tyrosine with mixture of 35Cl and 37Cl)) |
| 70 | Pyruvate |
| 71 | Sarcosyl |
| 71 | Alanyl (-A-, -Ala-) |
| 71 | Acetamidomethyl (Acm) |
| 71 | Propionamide or Acrylamide adduct |
| 72 | 3,5-Dichlorination (of Tyrosine with 37Cl) |
| 74 | S-(sn-1-Glyceryl) (on Cysteine) |
| 74 | Glycerol Ester (on Glutamic acid side chain) |
| 75 | Glycine (G, Gly) |
| 76 | Phenyl ester (OPh) (on acidic) |
| 76 | Beta mercaptoethanol adduct |
| 78 | 3-Bromination (of Tyrosine with 79Br) |
| 78 | L-o-bromination of Phe with 79Br |
| 80 | L-o-bromination of Phe with 81Br |
| 80 | Sulphonation (SO3H) (of PMC group) |
| 80 | Sulphation (of O of Tyrosine) |
| 80 | Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) |
| 80 | 3-Bromination (of Tyrosine with 81Br) |
| 82 | Cyclohexyl ester (OcHex) |
| 83 | Dehydroamino butyric acid (Dhb) |
| 83 | Homoseryl lactone |
| 85 | 2-Aminobutyric acid (Abu) |
| 85 | 2-Aminoisobutyric acid (Aib) |
| 85 | Gamma Aminobutyryl |
| 86 | t-butyloxymethyl (Bum) |
| 86 | Diaminopropionyl |
| 87 | N-(4-NH2-2-OH-butyl)- (of Lysine) (Hypusine) |
| 87 | Seryl (-S-, -Ser-) |
| 88 | t-butylsulfenyl (StBu) |
| 89 | Alanine (A, Ala) |
| 89 | Sarcosine (Sar) |
| 90 | Anisyl |
| 90 | Benzyl (Bzl) and benzly ester (OBzl) |
| 93 | 1,2-ethanedithiol (EDT) |
| 95 | Dehydroprolyl |
| 96 | Trifluoroacetyl (TFA) |
| 97 | N-hydroxysuccinimide (ONSu, OSu) |
| 97 | Prolyl (-P-, -Pro-) |
| 98 | Cysteic acid x2, oxidation of cystine |
| 98 | Tetramethylguanidinium termination by-product on amine |
| 98 | Phosphate/sulphate adduct of proteins |
| 99 | Valyl (-V-, -Val-) |
| 99 | Isovalyl (-I-,-Iva-) |
| 100 | t-Butyloxycarbonyl (tBoc) |
| 101 | Threoyl (-T-, -Thr-) |
| 101 | Homoseryl (-Hse-) |
| 103 | Cystyl (-C-, -Cys-) |
| 104 | 4-Methylbenzyl (Meb) |
| 104 | Benzoyl (Bz) |
| 105 | Serine (S, Ser) |
| 105 | Pyridylethylation of cysteine |
| 106 | HMP (hydroxymethylphenyl) linker |
| 106 | Thioanisyl |
| 106 | Thiocresyl |
| 111 | 2-Piperidinecarboxylic acid (Pip) |
| 111 | Pyroglutamyl |
| 113 | Hydroxyprolyl (-Hyp-) |
| 113 | Isoleucyl (-I-, -Ile-) |
| 113 | Leucyl (-L-, -Leu-) |
| 113 | Norleucyl (-Nle-) |
| 114 | Asparagyl (-N-, -Asn-) |
| 114 | t-amyloxycarbonyl (Aoc) |
| 114 | Ornithyl (-Orn-) |
| 115 | Proline (P, Pro) |
| 115 | Aspartyl (-D-, -Asp-) |
| 117 | Succinyl |
| 117 | Valine (V, Val) |
| 117 | Hydroxybenzotriazole ester (HOBt) |
| 118 | Dimethylbenzyl (diMeBzl) |
| 119 | Threonine (T, Thr) |
| 119 | Cysteinylation |
| 120 | Benzyloxymethyl (Bom) |
| 120 | p-methoxybenzyl (Mob, Mbzl) |
| 121 | 4-Nitrophenyl, p-Nitrophenyl (ONp) |
| 121 | Cysteine (C, Cys) |
| 125 | Chlorobenzyl (ClBzl) |
| 126 | Iodination (of Histidine[C4] or Tyrosine[C3]) |
| 128 | Glutamyl (-Q-, -Gln-) |
| 128 | Lysyl (-K-, -Lys-) |
| 129 | Glutamyl (-E-, -Glu-) |
| 129 | O-Methyl Aspartamyl |
| 130 | N alpha -(gamma-Glutamyl)-Glu |
| 131 | Norleucine (Nle) |
| 131 | Hydroxyproline (Hyp) |
| 131 | Isoleucine (I, Ile) |
| 131 | Leucine (L, Leu) |
| 131 | Methionyl (-M-, -Met-) |
| 131 | Hydroxy Aspartamyl |
| 131 | bb-dimethyl Cystenyl |
| 132 | Asparagine (N, Asn) |
| 132 | Pentoses (Ara, Rib, Xyl) |
| 133 | Aspartic Acid (D, Asp) |
| 134 | Benzyloxycarbonyl (Z) |
| 134 | Adamantyl (Ada) |
| 135 | p-Nitrobenzyl ester (ONb) |
| 137 | Histidyl (-H-, -His-) |
| 142 | N-methyl Glutamyl |
| 142 | N-methyl Lysyl |
| 143 | O-methyl Glutamyl |
| 143 | Diphthamide (from Histidine) |
| 144 | Hydroxy Lysyl (-Hyl-) |
| 145 | Methyl Methionyl |
| 146 | Glutamine (Q, Gln) |
| 146 | Deoxyhexoses (Fuc, Rha) |
| 146 | Lysine (K, Lys) |
| 146 | Pentosyl |
| 146 | Aminoethyl Cysteinyl (AECys) |
| 147 | 4-Glycosyloxy- (pentosyl,C5) (of Proline) |
| 147 | Glutamic Acid (E, Glu) |
| 147 | Phenylalanyl- (-F-, -Phe-) |
| 147 | Methionyl Sulfoxide |
| 148 | Pyridyl Alanyl |
| 149 | 2-Nitrobenzoyl (NBz) |
| 149 | Methionine (M, Met) |
| 149 | Fluorophenylalanyl |
| 150 | Dimethoxybenzyl Trp |
| 153 | 2-Nitrophenylsulphenyl (Nps) |
| 154 | 4-Toluenesulphonyl (Tosyl, Tos) |
| 154 | 3-nitro-2-pyridinesulfenyl (Npys) |
| 155 | Histidine (H, His) |
| 156 | 3,5-Dibromination (of Tyrosine with 79Br) |
| 156 | Arginyl (-R-, -Arg-) |
| 157 | Citrulline |
| 158 | 3,5-Dibromination (of Tyrosine with mixture of 79Br and 81Br) |
| 159 | Dichlorobenzyl (Dcb) |
| 160 | 3,5-Dibromination (of Tyrosine with 81Br) |
| 160 | Carboxyamidomethyl Cystenyl |
| 161 | Hexosamines (GalN, GlcN) |
| 161 | Carboxymethyl cysteine (Cmc) |
| 161 | Carboxymethyl Cystenyl |
| 161 | Methylphenylalanyl |
| 162 | Inositol |
| 162 | N-Glucosyl (N terminus or N epsilon of Lysine) (Aminoketose) |
| 162 | O-Glycosyl- (to Serine or Threonine) |
| 162 | Linker attached to peptide in Fmoc peptide synthesis |
| 162 | Hexoses (Fru, Gal, Glc, Man) |
| 163 | Tyrosinyl (-Y-, -Tyr-) |
| 163 | MethionylSulphone |
| 165 | Phenylalanine (F, Phe) |
| 166 | 2,4 -dinitrophenyl (Dnp) |
| 166 | Pentaflourophenyl (Pfp) |
| 166 | Diphenylmethyl (Dpm) |
| 167 | Phospho Seryl |
| 169 | 2-Chlorobenzyloxycarbonyl (ClZ) |
| 169 | Napthyl acetyl |
| 170 | N-acetyl Lysyl |
| 170 | N-methyl Arginyl |
| 172 | Ethanedithiol/TFA cyclic adduct |
| 173 | Carboxy Glutamyl (Gla) |
| 174 | Arginine (R, Arg) |
| 174 | Acetamidomethyl Cystenyl |
| 174 | Acrylamidyl Cystenyl |
| 176 | N-Glucuronyl (N terminus) |
| 177 | delta-Glycosyloxy- (of Lysine) or beta-Glycosyloxy- (of Phenylalanine or Tyrosine) |
| 177 | 4-Glycosyloxy- (hexosyl,C6) (of Proline) |
| 177 | Benzyl Seryl |
| 177 | N-methyl Tyrosinyl |
| 178 | a-N-Gluconoylation (His Tagged proteins) |
| 179 | p-Nitrobenzyloxycarbonyl (4Nz) |
| 179 | 2,4,5-Trichlorophenyl |
| 180 | 2,4,6-trimethyloxybenzyl (Tmob) |
| 180 | Xanthyl (Xan) |
| 181 | Tyrosine (Y, Tyr) |
| 182 | Chlorophenylalanyl |
| 182 | Mesitylene-2-sulfonyl (Mts) |
| 183 | AEBSF |
| 184 | Isopropyl Lysyl |
| 186 | Tryptophanyl (-W-, - Trp-) |
| 186 | Carboxymethyl Lysyl |
| 188 | N-Lipoyl- (on Lysine) |
| 190 | Matrix alpha cyano MH+ |
| 191 | Benzyl Threonyl |
| 193 | Benzyl Cystenyl |
| 197 | Napthyl Alanyl |
| 198 | Succinyl Aspartamyl |
| 201 | HMP (hydroxymethylphenyl)/TFA adduct |
| 203 | N-acetylhexosamines (GalNAc, GlcNAc) |
| 204 | Tryptophan (W, Trp) |
| 204 | Cystine ((Cys)2) |
| 204 | Farnesylation |
| 206 | S-Farnesyl- |
| 206 | Myristoylation-4H (2 double bonds) |
| 208 | Myristoleylation (myristoyl with one double bond) |
| 208 | Pyridylethyl Cystenyl |
| 210 | Myristoylation |
| 212 | 4-Methoxy-2,3,6-trimethylbenzenesulfonyl (Mtr) |
| 213 | 2-Bromobenzyloxycarbonyl (BrZ) |
| 214 | Formyl Tryptophanyl |
| 219 | Benzyl Glutamyl |
| 219 | Anisole Adducted Glutamyl |
| 222 | 9-Fluorenylmethyloxycarbonyl (Fmoc) |
| 222 | S-cystenyl Cystenyl |
| 226 | Biotinylation (amide bond to lysine) |
| 226 | Dimethoxybenzhydryl (Mbh) |
| 229 | N-Pyridoxyl (on Lysine) |
| 231 | Pyridoxal phosphate (Schiff Base formed to lysine) |
| 233 | Dansyl (Dns) |
| 233 | Nicotinyl Lysyl |
| 238 | 2-(p-biphenyl)isopropyl-oxycarbonyl (Bpoc) |
| 238 | Palmitoylation |
| 242 | Triphenylmethyl (Trityl, Trt) |
| 243 | Tyrosinyl Sulphate |
| 243 | Phospho Tyrosinyl |
| 252 | Pbf (pentamethyldihydrobenzofuransulfonyl) |
| 252 | 3,5-Diiodination (of Tyrosine) |
| 258 | a-N-6-Phosphogluconoylation (His Tagged proteins) |
| 259 | N alpha -(gamma-Glutamyl)-Glu2 |
| 266 | O-GlcNAc-1-phosphorylation (of Serine) |
| 266 | Stearoylation |
| 266 | Pmc (2,2,5,7,8- Pentamethylchroman-6-sulphonyl) |
| 272 | Geranylgeranylation |
| 272 | Monomethoxytrityl |
| 276 | S-Geranylgeranyl |
| 289 | 5'phos dCytidinyl |
| 289 | iodo Tyrosinyl |
| 290 | Aldohexosyl Lysyl |
| 291 | Sialyl |
| 291 | N-acetylneuraminic acid (Sialic acid, NeuAc, NANA, SA) |
| 304 | 5'phos dThymidinyl |
| 305 | 5'phos Cytidinyl |
| 305 | Glutathionation |
| 306 | O-Uridinylylation (of Tyrosine) |
| 306 | 5'phos Uridinyl |
| 307 | N-glycolneuraminic acid (NeuGc) |
| 307 | S-farnesyl Cystenyl |
| 313 | 5'phos dAdenosyl |
| 324 | O-pantetheinephosphorylation (of Serine) |
| 327 | SucPhencarb Lysyl |
| 329 | 5'phos dGuanosyl |
| 329 | 5'phos Adenosinyl |
| 329 | O-5'-Adenosylation ( of Tyrosine) |
| 339 | 4'-Phosphopantetheine |
| 342 | S-palmityl Cystenyl |
| 345 | 5'phos Guanosyl |
| 354 | Biotinyl Lysyl |
| 359 | Fluorescein labelling of peptide N-terminal using NHS ester |
| 365 | Hex-HexNAc |
| 388 | N alpha -(gamma-Glutamyl)-Glu3 |
| 391 | Dioctyl Phthalate |
| 395 | PMC Lysyl |
| 409 | Aedans Cystenyl |
| 413 | Dioctyl Phthalate Sodium Adduct |
| 415 | di-iodo Tyrosinyl |
| 423 | PMC Arginyl |
| 454 | S-Coenzyme A |
| 457 | AMP Lysyl |
| 470 | 3,5,3'-Triiodothyronine (from Tyrosine) |
| 524 | S-(sn-1-Dipalmitoyl-glyceryl)- (on Cysteine) |
| 541 | S-(ADP-ribosyl)- (on Cysteine) |
| 541 | N-(ADP-ribosyl)- (on Arginine) |
| 541 | O-ADP-ribosylation (on Glutamate or C terminus) |
| 541 | ADP-rybosylation (from NAD) |
| 587 | S-Phycocyanobilin (on Cysteine) |
| 617 | S-Heme (on Cysteine) |
| 648 | N theta -(ADP-ribosyl) diphthamide (of Histidine) |
| 657 | NeuAc-Hex-HexNAc |
| 783 | O-8 alpha-Flavin [FAD])- (of Tyrosine) |
| 784 | S-(6-Flavin [FAD])- (on Cysteine) |
| 784 | N theta and N pi-(8alpha-Flavin) (on Histidine) |
| 893 | (Hex)3-HexNAc-HexNAc |
| 1,039 | (Hex)3-HexNAc-(dHex)HexNAc |
